Glycomics and Glycoproteomics of Biological Systems
Lecturers: Anne Dell, Daniel Kolarich, Manfred Wuhrer,
This course will provide both historical perspectives and up-to-date insights into state-of-the-art methodologies for defining glycomes and glycoproteomes in biological systems. Topics will include choices of workflows, glycomic and glycoproteomic mapping, fragmentation pathways, imaging, high-throughput analyses, manual and automated data interpretation.
|09:10-09:50||Anne Dell (Imperial College London, UK): Reflections on the history and current status of systems glycomics|
|10:00-10:40||Daniel Kolarich (Griffith University, Australia): The Hitchhiker's guide to glycoproteomics|
|11:10-11:50||Manfred Wuhrer (Leiden University, Netherlands): Intact proteins, high-throughput, and spatial glycomics|
Title: Reflections on the history and current status of systems glycomics
Much of glycobiology research seeks to understand how glycans on glycoproteins, glycolipids and lipopolysaccharides engage with glycan-binding proteins to mediate adhesive and signalling events. Such recognition is central to cell-cell communication including, for example, interactions between mammalian eggs and sperm, between pathogens, parasites and their hosts, between cells of the immune system, and between the microbiome and the gastro-intestinal and cervicovaginal tracts. In partnership with bioscience collaborators worldwide, my laboratory at Imperial College London specialises in the development and exploitation of high sensitivity mass spectrometry with the aim of establishing the roles that glycans play in health and disease. Drawing on case studies from over forty years of collaborative research, this lecture will provide both a historical perspective and an assessment of the current status of high sensitivity glycomic methodologies for defining N- and O-glycomes of cells, tissues, organs and biological fluids.
Title: The Hitchhiker's guide to glycoproteomics
Glycoproteomics, the study of glycans and their carrier proteins in a system-wide context, is becoming a powerful tool in glycobiology that enables the functional analysis of protein glycosylation. This presentation will provide a concise overview on the approaches, methodologies, and technologies that are relevant within a glycoproteomics experiment, from the start to the data analysis and interpretation. We will discuss the challenges, limitations but also unique opportunities related to the common strategies employed to capture glycoprotein-specific and system- wide glycoproteome data from tissues, body fluids, or cells, and provide a perspective on how integration into multi-omics workflows translates into new knowledge in glycobiology and its role in health and disease.
Title: Intact protein, high-throughput, and spatial glycomics
Mass spectrometry imaging of N-glycans discloses cell-type and tissue-specific glycosylation in cancer and inflammatory conditions. Native mass spectrometry, in conjunction with affinity capillary electrophoresis and affinity chromatography is a powerful approach for exploring the role of glycosylation in modulation of protein interactions. Moreover, by analyzing glycosylation from hundreds to thousands of clinical samples the link of glycosylation to disease and genomic, metabolomic and immunological markers can be established.